DIRECTIONS OF TESTS
The priority directions of scientific research carried out in the Department of Neurology include research on the assessment of the importance of intercellular communication through exosomes as a mechanism for the development of damage to distant centers after a trauma to the central nervous system (CNS). Models tested include traumatic brain injury (TBI) and autoimmune focal encephalitis - experimental autoimmune encephalomyelitis (EAE). EAE is an animal model of multiple sclerosis (MS).
The department analyzes exosomes isolated from serum and plasma. The quantitative and qualitative parameters of exosomes, such as their concentration, size, protein content and RNA are evaluated. Exosome protein profile studies are performed using proteonomic analysis. In particular, the presence of myelin proteins, myelin basic protein (MBP), proteolipid protein (PLP), oligodendrocyte protein (MOG) and other CNS proteins is sought. Using the NGS sequencing technique, the presence of transcripts encoding proteins and lncRNA (long non-coding RNA) is also assessed.